The documented dataset covers Enterprise Survey (ES) panel data collected in Nicaragua in 2003, 2006, 2010 and 2016, as part of Latin America and the Caribbean Enterprise Surveys rollout, an initiative of the World Bank. The objective of the survey is to obtain feedback from enterprises on the state of the private sector as well as to help in building a panel of enterprise data that will make it possible to track changes in the business environment over time, thus allowing, for example, impact assessments of reforms. Through interviews with firms in the manufacturing and services sectors, the survey assesses the constraints to private sector growth and creates statistically significant business environment indicators that are comparable across countries. Only registered businesses are surveyed in the Enterprise Survey. Enterprise Surveys target a sample consisting of longitudinal (panel) observations and new cross-sectional data. Panel firms are prioritized in the sample selection, comprising up to 50% of the sample. For all panel firms, regardless of the sample, current eligibility or operating status is determined and included in panel datasets. Nicaragua ES 2010 was conducted in August 2010- May 2011, Ecuador ES 2016 was carried out in October 2016 June 2017. Stratified random sampling was used to select the surveyed businesses. Data was collected using face-to-face interviews. Data from 1,599 establishments was analyzed: 211 businesses were from 2003 only, 153 firms were from 2006 only, 119 from 2010 only, 213 from 2016 only, 146 firms were from 2010 and 2016, 110 from 2006 and 2010, 72 firms were from 2003, 2006, 2010 and 2016.